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KMID : 1094720180230050580
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Biotechnology and Bioprocess Engineering
2018 Volume.23 No. 5 p.580 ~ p.587
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Intergeneric Hybridization between Streptomyces albulus and Bacillus subtilis Facilitates Production of ε-Poly-L-lysine from Corn Starch Residues
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Li Shu
Wang Nan
Du Zong-Jun
Chen Guan-Jun
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Abstract
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Intergeneric hybridization between S.albulus and B. subtilis to produce ε-poly-L-lysine (ε-PL) from corn starch residues (CSR) was investigated in this study. One hybrid, designated S. albulus LS-84, which incorporated the protease gene from B. subtilis, could effectively utilize the protein in CSR as a nitrogen source. In fed-batch fermentation, LS-84 produced 32.6 g/L ε-PL in the presence of 20 g/L CSR. This was an increase of 256.1% compared to that of the parent strain S. albulus LS-01. The rapid hydrolysis of CSR by protease caused rapid growth for LS-84, which allowed higher respiratory activity. As a result, activities of several key enzymes in LS-84 were higher than those in LS-01; additionally, the content of several intracellular amino acids, such as Asp, Glu, and Arg, was also much higher in LS-84. Therefore, intergeneric hybridization between S. albulus and B. subtilis to produce ε-PL from CSR is an economical method for effective utilization of waste resources.
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KEYWORD
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ε-poly-L-lysine, corn starch residues, intergeneric hybridization
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